ROR1 (DM149) Rabbit mAb

Name: ROR1 (DM149) Rabbit mAb
SKU: 28493
Price: 565.00 USD
Availability: InStock

$565.00

Cat.#: 28493

Product Name: Anti-ROR1(DM149) Rabbit Monoclonal Antibody

Synonyms: ROR1;NTRKR1

Description: Anti-ROR1 antibody(DM149) Rabbit Monoclonal Antibody

Background: This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq; Jun 2012]

Applications: ELISA; Flow Cyt

Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100

Host Species: Rabbit

Isotype: Rabbit IgG

Purification: Purified from cell culture supernatant by affinity chromatography

Species Reactivity: Human ROR1

Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.

Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human ROR1 protein, His tagged protein 11267 can bind Rabbit anti-ROR1 monoclonal antibody (clone: DM149) in a linear range of 1-50 ng/ml.

Figure 2. ROR1 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-ROR1 (DM149) on Expi293 cells transfected with human ROR1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).