Protein expression and purification in E. coli, mammalian, and yeast systems — from gene optimization to purified protein.
E coli Protein Service
We offer multiple fusion/affinity tags to increase the protein solubility, expression levels, and ease of purification.
The advantages of E. coli expression systems include easy operation, low cost, high expression, as well as in-depth research on its genetic background and the diversity of vector strain selection, the use of E. coli to overexpress target genes is still the preferred method at present. However, the codon preferences of different species and the cytotoxicity of expressed products pose a significant challenge to protein expression. Common methods to solve these problems include codon optimization, adjustment of induction conditions, and co-expression of chaperone proteins. In addition, the fusion expression of target protein and soluble tag can significantly improve the solubility of target protein.
Highlights
- High Success Rate – To provide soluble protein with an accuracy rate of 95%
- Optional Expression Vectors – More than 10 conventional or optimized vectors can be selected.
- Optional Protein Tags – More than 10 commonly used fusion tags like SUMO, Trx, GST, NusA, etc.
- Optional Expression Hosts – More than 10 E. coli hosts available for selection.
- Large-scale Production – Scale 1-4,000 L per batch.
- One-stop Service – From Cloning to Purification.
Process
| Process | Contents | Timeline |
|---|---|---|
| Plasmid Construction | Gene synthesis / codon optimization, vector construction | 2 Weeks |
| Protein Expression Test | Different vectors and hosts are tested to find the best expression system | 3 Weeks |
| Large-Scale Fermentation & Purification | According to the experimental conditions established by the pilot test, enlarged fermentation is carried out, and protein purification is carried out, including affinity chromatography and ion exchange, etc. | 3-5 Weeks |
Mammalian Protein Expression Service
We are an expert on CHO and HEK293 cell expression. We can meet your special needs such as serum-free culture, tag excision, endotoxin removal, and mass spectrometry analysis.
Mammalian protein expression system is an important tool in biological research, drug development and industrial production. Compared with the prokaryotic expression system, it has more complex post-translation modification ability, which can make the recombinant proteins have the correct folding, processing and activity. CHO (Chinese hamster ovary) cells and HEK293 (human embryonic kidney) cells are two commonly used mammalian expression systems. CHO cells have become the preferred cell line for industrial production due to their high yield, low cost and good stability. HEK293 cells are widely used in basic research and drug screening because of their high transfection efficiency and easy culture. Our company provides expression and purification services of mammalian protein expression system to help you quickly obtain high-quality protein.
Highlights
- High activity and high success rate – Proteins expressed in mammalian cells can achieve complex post-translational modifications.
- Multiple expression system options – Transient transfection expression; Stable transfection expression; Recombinant antibody expression, and preparation
- One-stop Service – Design and optimize gene; Optimize protein expression conditions; Purify protein and post processing; Manage data and report.
Stable Cell Line Construction
| Step | Contents | Timeline |
|---|---|---|
| Vector Design | Conducts bioinformatic analysis on target(s) and recommends an expression strategy to each client. | 1 Week |
| Plasmid Construction | Gene optimization and synthesis, Vector construction | 2-3 Weeks |
| Identification of polyclonal stable cell lines | Vector transformation, screening and identification of positive cell clones | 2-3 Weeks |
| Screening of monoclonal stable cell lines | Cell subclonal screening to screen out monoclonal cell lines with high expression levels and stable performance | 3-4 Weeks |
| Stable cell line quality test | Perform performance verification according to customer requirements, such as protein activity, expression level, yield, etc. | 1-2 Weeks |
Yeast Protein Expression Services
Yeast expression system is one of the most economical eukaryotic expression system for scale up production of both secreted and intracellular proteins that require post-translational modifications.
Yeast protein expression system is the most economical and efficient expression system among eukaryotic protein expression systems, which combines the advantages of prokaryotic expression system and eukaryotic expression system. Because of its fast growth rate, low culture cost and clear genetic background, it can provide goods with high efficiency and low cost, and its application range can cover many fields such as food, biopharmaceuticals, and scientific research.
Highlights
- High activity and high success rate – According to the codon preference, to optimize the target gene, Effectively improve protein solubility and expression.
- Multi-carrier selection scheme – According to the characteristics of different proteins Vectors suitable for extracellular secretion and intracellular expression were selected
- Optimization of induction conditions – By optimization of Conditions such as methanol concentration and induction time, we can effectively improve protein expression.
- Extra Services – Tag removal; Endotoxin removal
Stable Cell Line Construction Service
| Step | Contents | Timeline |
|---|---|---|
| Vector Design | Conducts bioinformatic analysis on target(s) and recommends an expression strategy to each client. | 1 Week |
| Plasmid Construction | Gene optimization and synthesis, Vector construction | 2-3 Weeks |
| Identification of polyclonal stable cell lines | Vector transformation, screening and identification of positive cell clones | 2-3 Weeks |
| Screening of monoclonal stable cell lines | Cell subclonal screening to screen out monoclonal cell lines with high expression levels and stable performance | 3-4 Weeks |
| Stable cell line quality test | Perform performance verification according to customer requirements, such as protein activity, expression level, yield, etc. | 1-2 Weeks |
Questions About Our Protein Services?
Email sale@neweastbio.com or call (610) 945-2007 — or see How to Order.