GITR (DM79) Rabbit mAb

Name: GITR (DM79) Rabbit mAb
SKU: 28399
Price: 565.00 USD
Availability: InStock

$565.00

Cat.#: 28399

Product Name: Anti-GITR(DM79) Rabbit Monoclonal Antibody

Synonyms: AITR; GITR; TNFRSF18; CD357

Description: Anti-GITR antibody(DM79) Rabbit Monoclonal Antibody

Background: This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation; and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25( )CD4( ) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Applications: ELISA; Flow Cyt

Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100

Host Species: Rabbit

Isotype: Rabbit IgG

Purification: Purified from cell culture supernatant by affinity chromatography

Species Reactivity: Human GITR

Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.

Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human GITR protein, hFc-His tagged protein 11146 can bind Rabbit anti-GITR monoclonal antibody (clone: DM79) in a linear range of 1-100 ng/ml.	Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human GITR (B) were surface stained with Rabbit anti-GITR monoclonal antibody 1µg/ml (clone: DM79) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry data of serially titrated Rabbit anti-GITR monoclonal antibody (clone: DM79) on PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.	Figure 4. Affinity ranking of different Rabbit anti-GITR mAb clones by titration of different concentration onto PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.