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CD34 (DM136) Rabbit mAb

$565.00

Cat.#:   28481
Product Name:   Anti-CD34(DM136) Rabbit Monoclonal Antibody
Synonyms:   CD34
Description:    Anti-CD34 antibody(DM136) Rabbit Monoclonal Antibody
Background:    The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human CD34
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human CD34 protein, His tagged protein 11280 can bind Rabbit anti-CD34 monoclonal antibody (clone: DM136) in a linear range of 0. 1-12 ng/ml.

Figure 2. Flow cytometry analysis with Anti-CD34  (DM136) on Expi293 cells transfected with human CD34(Blue histogram) or Expi293 transfected with irrelevant protein(Red histogram).

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