B7-2 (DM84) Rabbit mAb

Name: B7-2 (DM84) Rabbit mAb
SKU: 28409
Price: 565.00 USD
Availability: InStock

$565.00

Cat.#: 28409

Product Name: Anti-B7-2(DM84) Rabbit Monoclonal Antibody

Synonyms: CD86; B7-2; B70; CD28LG2; LAB72; MGC34413

Description: Anti-B7-2 antibody(DM84) Rabbit Monoclonal Antibody

Background: This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells; and it is the ligand for two proteins at the cell surface of T cells; CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.

Applications: ELISA; Flow Cyt

Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100

Host Species: Rabbit

Isotype: Rabbit IgG

Purification: Purified from cell culture supernatant by affinity chromatography

Species Reactivity: Human B7-2

Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.

Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-2 protein, mFc-His tagged protein 11162 can bind Rabbit anti-B7-2 monoclonal antibody (clone: DM84) in a linear range of 1-100 ng/ml.

Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human B7-2 (B) were surface stained with Rabbit anti-B7-2 monoclonal antibody 1µg/ml (clone: DM84) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.