HDL
$350.00
Name:2.0 mg
| Cat. #: | 10460 | |
| Purity: | 98% (Co-migrates with reference on agarose gel electrophoresis) | |
| Concentration: | Minimum 2.25 mg/ml protein | |
| Description: | Human High Density Lipoprotein | |
| Background:
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LDL is a large protein (MW 3,500 kDa) with a diameter of 25.8 nm. It is composed of approximately 20-25% protein and 75-80% lipid. The lipid portion can be further described as 9% free cholesterol, 42% cholesteryl ester, 20-24% phospholipid, and 5% triglyceride.
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| Source: | Human plasma, which tested negative for HBsAG, HIV1/2, HIV-NAT, HCV, HCV-NAT and Syphilis. It is purified via ultracentrifugation to homogeneity (d=1.063～1.21 g/mL) determined by agarose gel electrophoresis. Each lot is analyzed on agarose gel electrophoresis for migration versus HDL. | |
| Tested Applications: | Human HDL are evaluated for receptor binding to Activity: peritoneal macrophages in conjunction with our DiI-HDL and [I-125] Ox-HDL. | |
| Storage & Stability: | Human HDL is stable for 6 weeks after receipt when handled aseptically and stored at 2-8°C (Don’t Freeze). Note: After prolonged storage, some precipitate may be observed. This is normal for the product. Spin in centrifugation at 1000×g for 3 minutes before using. | |
| Packaging: | HDL is membrane filtered (0.22 micron) and aseptically packaged under nitrogen in a solution containing phosphate-buffered saline at pH 7.4 and 0.2 mM EDTA-Na2. The product requires 1 week lead time. Please plan your experiments in advance and use the fresh material. | |