CTC-BIOPSY®-A10 — Automatic Circulating Tumor Cell Separator
Based on the physical differences such as size, deformability, and fluid dynamics between Circulating Tumor Cells (CTCs, 1 ctc per ml blood) and blood cells, the whole blood is filtered through patented nano-microsieve membrane and then CTCs with cell diameter over 15 µm are captured and enriched on the membrane.
Cat. #: CTC-BIOPSY-A10
Applications
Circulating Tumor Cell (CTC) detection offers advantages such as non-invasive, real-time monitoring.
CTCs can be thought of as a liquid biopsy from the blood. The clinical applications of CTCs include early screening, diagnosis, targeted therapy, post-treatment assessment, identification of tumor DNA mutations, or monitoring minimal residual disease (MRD) or indicates poorer prognosis.
Mechanism — Separation by Filtration
Utilizing suction filtration technique to separate CTC and CTM from the red blood cells in the whole blood.
The membrane is nano-microsieve with 13 mm in diameter. It has 160,000 micropores. The pore size is 8 µm.
The micro sieve membrane is second-generation nano polymer material. It is resistance to various stains, does not require pre-treatment by inactivated plasma free or whole cells, and does not depend on the morphology of the cells.
Operation
High automation, 10 mins to complete CTC capture and enrichment of 5ml of the whole blood.
Nano-microscreen technology, no reliance on specific cell marker, suitable for all types of tumors.
CTC Classifications
- CTC – Circulating Tumor Cells in peripheral blood, single cells or microclusters, and can be combined with platelets, endothelial cells, etc. to produce CTM.
- CTM – Circulating Tumor Microemboli (CTM) are clusters of 3 or more CTCs in the blood. For CTM, the risk of death of patients after detection is increased by 23-50 times compared with CTC alone, and the risk of metastasis is increased by 23-50 times.
- Neutrophil CTM – CTM that has been associated with neutrophils is referred to as Neutrophil CTM. The risk of death for patients with Neutrophil CTM is increased by 62 times compared with CTC alone, and the survival period is decreased by 5 times.
Risk order: CTC < CTM < Neutrophil CTM
CTC Rarity
1 per ml of the Whole Blood
Morphological Criteria for CTC Identification
- Cell diameter > 15 µm
- Nucleus/cytoplasm ratio > 0.8
- Irregular nuclear shape
- Thickened nuclear membrane with visible heterochromatin
- Large nucleoli (especially in dividing cells)
- More than 4 cells in a cluster / overlapping cell clusters
Scholars believe that a decrease of more than 4 points in the CTC criterion is of diagnostic significance.
If you find Claudin+- or CK19+ cells, they are also tumor cells.
If a cell is larger than 60 µm and has a prominent nucleolus in hyperchromatic thick nucleus, this is be a ‘CTC cluster’.
CTC pathology detection is the gold standard – classic technology.
Downstream Applications
- IHC
- IF
- FISH
- Cell Culture
Questions about the CTC Separator? Email sale@neweastbio.com or call (610) 945-2007.
A Solution for Real-time Tumor Monitoring — Convenient · Efficient · Accurate
CTC-BIOPSY®-A10