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GPRC5D (DM61) Rabbit mAb

$565.00

Cat.#:   28365
Product Name:   Anti-GPRC5D(DM61) Rabbit Monoclonal Antibody
Synonyms:   GPRC5D
Description:    Anti-GPRC5D antibody(DM61) Rabbit Monoclonal Antibody
Background:    The protein encoded by this gene is a member of the G protein-coupled receptor family; however; the specific function of this gene has not yet been determined.
Applications:    ELISA; Flow Cyt; IHC
Recommended Dilutions:    IHC 1:200, ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human GPRC5D
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human GPRC5D protein, hFc-His tagged protein ([getskuurl sku11189 can bind Rabbit anti-GPRC5D monoclonal antibody ( clone: DM61) in a linear range of 1-100 ng/ml.

Figure 2. Expi 293 cell line transfected with irrelevant protein  (A) and human GPRC5D  (B) were surface stained with Rabbit anti-GPRC5D monoclonal antibody 15µg/ml ( clone: DM61) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Figure 4. Affinity ranking of different Rabbit anti- GPRC5D mAb clones by titration of different concentration onto H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Figure 5. IHC staining of H929 cells A. with rabbit anti-GPRC5D mAb (Cat# 28365) at 5.9 ug/ml.  B. with another anti-GPRC5D mAb (Cat# 28421) at 8.8 ug/ml. 28365 can specifically react with GPRC5D in IHC application.

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