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CD30 (DM103) Rabbit mAb

$565.00

Cat.#:   28448
Product Name:   Anti-CD30(DM103) Rabbit Monoclonal Antibody
Synonyms:   TNFRSF8;CD30;D1S166E;Ki-1
Description:    Anti-CD30 antibody(DM103) Rabbit Monoclonal Antibody
Background:    The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated; but not by resting; T and B cells. TRAF2 and TRAF5 can interact with this receptor; and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis; and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human CD30
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD30 protein, His tagged protein 11291 can bind Rabbit anti-CD30 monoclonal antibody  (clone: DM103) in a linear range of 0.12-60 ng/ml.

Figure 2. Flow cytometry analysis with Anti-CD30  (DM103) on Expi293 cells transfected with human CD30(Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

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