PD-L1 (DM123) Rabbit mAb

Name: PD-L1 (DM123) Rabbit mAb
SKU: 28468
Price: 565.00 USD
Availability: InStock

$565.00

Cat.#: 28468

Product Name: Anti-PD-L1(DM123) Rabbit Monoclonal Antibody

Synonyms: PD-L1; CD274; B7-H1; PDCD1L1; PDCD1LG1

Description: Anti-PD-L1 antibody(DM123) Rabbit Monoclonal Antibody

Background: This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells; such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue; this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments; this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies; including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.

Applications: ELISA; Flow Cyt

Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100

Host Species: Rabbit

Isotype: Rabbit IgG

Purification: Purified from cell culture supernatant by affinity chromatography

Species Reactivity: Human PD-L1

Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.

Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human PD-L1 protein, mFc His tagged protein 11151 can bind Rabbit anti-PD-L1 monoclonal antibody (clone: DM123) in a linear range of 0.1-15 ng/ml.

Figure 2. Flow cytometry analysis with Anti-PD-L1 (DM123) on Expi293 cells transfected with human PD-L1 (Blue histogram) or Expi293 transfected with irrelevant protein(Red histogram).